HIGHLY LOCALIZED SUPERFUSION DEVICE FOR ADVANCED SINGLE-CELL EXPERIMENTS
The ability to study and interrogate one or a small number of cells requires precision control over the environment immediately surrounding a cell. The BioPen is a unique and powerful microfluidic system for maintaining localized superfusion in single-cell experiments, enabling control of the chemical environment around single intact cells in tissue or cell cultures.
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Interrogate single cells
Expose just one or a small number of cells to particular test solutions, e.g. drugs or antibodies, without diffusion outside the experimental region of interest. The test solution can easily be confined.
Deliver four different solutions to just a single cell or part of a cell with sub-second exchange time.
Low compound consumption
The four integrated solution reservoirs containing less than 35 μl, overcome typical dead volume problem of external tubing, reducing significantly the use of expensive reagents.
Combination with other probing devices
The pipette tip has been optimized to fit into practically any micromanipulation environment allowing combination with other probes such as patch-clamp pipettes, optical fibers or electrodes.
No glass or tip breakage
The pipette tips are made from a flexible biocompatible material so that unlike glass pipettes they won’t break upon contact with the hard surface.
The pipette tip has been developed from an hydrodynamically confined flow principle, enabling contamination-free confinement of one miscible liquid inside another.
The BioPen Microfluidic System is comprised of a high precision pressure controller, a disposable pipette tip inserted in a holder and, PC software. It integrates with common micromanipulators and microscopes.
The BioPen system contains:
1 – Disposable pipette tip:
The pipette tip is used to deliver up to 4 different solutions. Utilizing hydrodynamic confinement, the delivered solution can be localized and rapidly switched without contaminating the rest of the cells or the cell dish solution. The pipette tip is made from a flexible medical grade elastomer material so that unlike glass pipettes it won’t break upon contact with a hard surface. It includes 8 wells; 4 wells for containing solutions and 4 wells for collecting waste. Each well can contain up to 35 μl of solution.
2 – Pressure Controller:
Two levels of pneumatic pressure are supplied by the pressure controller and fast solution switching is achieved through the use of miniature solenoid valves.
3 – Pipette holder:
The pipette holder is a pressure manifold to assure a closed system and to connect each solution reservoir of the pipette tip individually with the precision pressure controller. It has been designed to be compatible with any standard type of micromanipulator such as, for example, the PatchStar from Scientifica.
4 – Software:
The software enables facile configuration and use of the BioPen system. The Windows-based BioPen software allows independent control of each pressure line and solution delivery. It also includes a macro scripting module for automation of common tasks and setup/shutdown. It can furthermore, be integrated into LabVIEW workflows.
|Dimensions||258 mm (W) × 170 mm (L) × 55 mm (H) (10 in. × 7 in. × 2 in.)|
|Weight||1.9 kg (4.19 Ib)|
|Cooling mode||Air cooled (natural convection)|
|Environmental conditions||For indoor laboratory use|
|Operating temperature||20°C to 30°C|
|Storage temperature||5°C to 35°C|
|Pneumatic connections||8 (4 for positive pressure, 4 for negative pressure)|
|Positive pressure range||0-450 mbar|
|Negative pressure range||-300-0 mbar|
|Pressure accuracy||± 5 mbar|
|Electricity specification||100-240 VAC 50/60 Hz|
|Electronic synchronization||5V TTL Out & In|
|Operating system||Microsoft Windows 10,8 and 7 (32 or 64 bit)|
|Hardware interface||USB 2.0 or higher|
Please download the full BioPen specifications and schematics.
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