Question 1

Can the flow be stopped and started again?

Celine Billerit · Accepted Answer

This is not a problem when the cell is in the collimated flow zones, where only the substance that flows out of the channel is present. But, the cells present in the recording chamber will be affected by the compounds which flow out. If the flow has been running during a whole scan it is advisable to add new fresh cells. Additionally,  the chamber should be rinsed thoroughly before applying new cells.

Question 2

Can the flow be stopped and started again?

Celine Billerit · Accepted Answer

This is not a problem when the cell is in the collimated flow zones, where only the substance that flows out of the channel is present. But, the cells present in the recording chamber will be affected by the compounds which flow out. If the flow has been running during a whole scan it is advisable to add new fresh cells. Additionally,  the chamber should be rinsed thoroughly before applying new cells.