Question 1

Is the cell negatively affected, seen from a physiological point of view, by long measurement times?

Celine Billerit · Accepted Answer

This does not differ from conventional patch clamp and depends to a large extent on which ion channel systems are studied. Some ion channels are reconstituted in membrane without loss of function. Others need an “intracellular cocktail” in the buffer to function properly.

Question 2

Is the cell negatively affected, seen from a physiological point of view, by long measurement times?

Celine Billerit · Accepted Answer

This does not differ from conventional patch clamp and depends to a large extent on which ion channel systems are studied. Some ion channels are reconstituted in membrane without loss of function. Others need an “intracellular cocktail” in the buffer to function properly.