BioPen® SYSTEMS

MICROFLUIDIC SYSTEMS FOR TARGETING SINGLE CELLS

Control the microenvironment of your cells by targeting one cell at the time

The ability to target one or a small number of cells requires precision control over the environment immediately surrounding a cell.

BioPen PRIME and BioPen FLEX systems are easy-to-use microfluidic devices for maintaining a local delivery of molecules to single-cells, enabling control of the chemical environment around single intact cells in tissue or cell cultures.

Contact Us to get more information about BioPen.

 

  • Target single cells

Target just one or a small number of adherent cells to particular compounds, e.g. drugs or antibodies, without diffusion outside the experimental region of interest. The compound can easily be confined.

  • Rapid delivery

Deliver four different compounds to just a single cell or part of a cell with sub-second exchange time.

  • Low compound consumption

The four integrated wells containing less than 35 μl, overcome typical dead volume problem of external tubing, reducing significantly the use of expensive reagents.

  • Combination with other probing devices

The pipette tip has been optimized to fit into practically any micromanipulation environment allowing combination with other probes such as patch-clamp pipettes, optical fibers or electrodes.

  • No glass or tip breakage

The pipette tips are made from a flexible biocompatible material so that unlike glass pipettes they won’t break upon contact with the hard surface.

  • No contamination

The pipette tip has been developed from an hydrodynamically confined flow principle, enabling contamination-free confinement of one miscible liquid inside another.

BioPen® PRIME and BioPen® FLEX Systems are comprised of a high precision pressure controller, a disposable pipette tip inserted in a holder and, PC software. They integrate with common micromanipulators and microscopes.

 

BioPen PRIME System

A system contains:

1 – Disposable pipette tip: 

The pipette tip is used to deliver up to 4 different compounds. The pipette tip is made from a flexible medical grade elastomer material so that unlike glass pipettes it won’t break upon contact with a hard surface. It includes 8 wells; 4 wells for containing compounds and 4 wells for collecting waste. Each well can contain up to 35 μl of drugs. Two types of tips are available: PRIME and FLEX.

Discover BioPen PRIME and FLEX Pipette tips >>

New  Sterile PRIME and FLEX pipette tips are now available!

2 – Pressure Controller:

Two levels of pneumatic pressure are supplied by the pressure controller and fast solution switching is achieved through the use of miniature solenoid valves.

3 – Pipette holder:

The pipette holder is a pressure manifold to assure a closed system and to connect each solution reservoir of the pipette tip individually with the precision pressure controller. It has been designed to be compatible with any standard type of micromanipulator such as, for example, the PatchStar from Scientifica. Two types of pipette holders are available: PRIME and FLEX.

Discover BioPen PRIME and FLEX Pipette Holders >>

4 – Software:

The software enables facile configuration and use of the BioPen® system. The Windows-based BioPen® software allows independent control of each pressure line and compounds delivery. It also includes a scripting module for automation of common tasks and setup/shutdown.

COMPARE BIOPEN PRIME AND BIOPEN FLEX

Find the best BioPen System for your application needs >>

 

Prime and Flex

BioPen tips

List of publications using BioPen

AuthorsTitleJournalYeardoi
Trkulja, Carolina L., Jungholm, Oscar, Davidson, Max, Jardemark, Kent, Marcus, Monica M., Hägglund, Jessica,Karlsson, Anders, Karlsson, Roger, Bruton, Joseph, Ivarsson, Niklas,Srinivasa, Sreesha P., Cavallin, Alexandra, Svensson, Peder, Jeffries, Gavin D. M., Christakopoulou, Maria-Nefeli, Reymer, Anna, Ashok, Anaswara, Willman, Gabriella, Papadia, Daniela, Johnsson, Emma, Orwar, OweRational antibody design for undruggable targets using kinetically controlled biomolecular probesScience Advances202110.1126/sciadv.abe6397
Karolina Spustova, Elif Senem Köksalm Alar Ainlam Irep GözenSubcompartmentalization and Pseudo‐Division of Model ProtocellsSmall202010.1002/smll.202005320
Jové, V., Zhongyan, G., Hol, F.J.H., Zhao, Z., Sorrells, T.R., Carroll, T.S., Prakash, M., McBride, C.S., Vosshall, L.B.Sensory Discrimination of Blood and Floral Nectar by Aedes aegypti MosquitoesNeuron202010.1016/j.neuron.2020.09.019
Köksal, E.S., Liese, S., Xue, L., Ryskulov, R., Viitala, L., Carlson, A., Gözen, I.Rapid Growth and Fusion of Protocells in Surface‐Adhered Membrane NetworksSmall202010.1002/smll.202002529
Perpiñá-Viciano, C., Işbilir, A., Zarca, A., Caspar, B., Kilpatrick, LE., Hill, SJ., Smit, MJ., Lohse, MJ., and Hoffmann, C.Kinetic Analysis of the Early Signaling Steps of the Human Chemokine Receptor CXCR4Molecular Pharmacology202010.1124/mol.119.118448
Jõemetsa, S., Spustova, K., Kustanovich, K., Ainla, A., Schindler, S., Eigler, S., . . . Gözen, I. Molecular Lipid Films on Microengineering Materials.Langmuir201910.1021/acs.langmuir.9b01120
Köksal, E., Liese, S., Kantarci, I., Olsson, R., Carlson, A., & Gözen, I.Nanotube-Mediated Path to Protocell Formation.ACS Nano201910.1021/acsnano.9b01646
Orwick Rydmark, M., Christensen, M., Köksal, E., Kantarci, I., Kustanovich, K., Yantchev, V., . . . Gözen, I. Styrene maleic acid copolymer induces pores in biomembranes.Soft Matter201910.1039/c9sm01407a
Markwardt, M., Snell, N., Guo, M., Wu, Y., Christensen, R., Liu, H., . . . Rizzo, M. A Genetically Encoded Biosensor Strategy for Quantifying Non-muscle Myosin II Phosphorylation Dynamics in Living Cells and Organisms. Cell Reports201810.1016/j.celrep.2018.06.088.
Wright, S., Consuelo, M., Cañizal, A., Benkel, T., Simon, K., Le Gouill, C., . . . Hoffmann, CFZD5 is a Gαq-coupled receptor that exhibits the functional hallmarks of prototypical GPCRsScience Signaling201810.1126/scisignal.aar5536
Oehler, B., Mohammadi, M., Viciano, C., Hackel, D., Hoffmann, C., Brack, A., & Rittner, H.Peripheral interaction of resolvin D1 and E1 with opioid receptor antagonists for antinociception in inflammatory pain in rats. Frontiers in Molecular Neuroscience201710.3389/fnmol.2017.00242
Waxse, B., Sengupta, P., Hesketh, G., Lippincott-Schwartz, J., & Buss, F. Myosin VI facilitates connexin 43 gap junction accretion. Journal of Cell Science201710.1242/jcs.199083
Gözen, I., Jeffries, G., Lobovkina, T., Celauro, E., Shaali, M., Doosti, B., & Jesorka, A. Single-Cell Analysis with the BioPenOpen Space Microfluidics (Book)201610.1002/9783527696789.ch10
Ahemaiti, A., Wigström, H., Ainla, A., Jeffries, G., Orwar, O., Jesorka, A., & Jardemark, K.Spatial characterization of a multifunctional pipette for drug delivery in hippocampal brain slices.Journal of Neuroscience Methods201510.1016/j.jneumeth.2014.12.017
Xu, S., Kim, A., Jeffries, G., & Jesorka, A.A rapid microfluidic technique for integrated viability determination of adherent single cells. Analytical and Bioanalytical Chemistry201510.1007/s00216-014-8364-9
Zhang, H., Kim, A., Xu, S., Jeffries, G., & Jesorka, A.Cellular communication via directed protrusion growth: Critical length-scales and membrane morphology. Nano Communication Networks201510.1016/j.nancom.2015.10.001
Zhang, H., Xu, S., Jeffries, G., Orwar, O. Artificial nanotube connections and transport of molecular cargo between mammalian cells.Nano Communication Networks201310.1016/j.nancom.2013.08.006
Bruton, J., Jeffries, G., & Westerblad, H.Usage of a localised microflow device to show that mitochondrial networks are not extensive in skeletal muscle fibres. PLoS ONE201410.1371/journal.pone.0108601
Ahemaiti, A., Ainla, A., Jeffries, G., Wigström, H., Orwar, O., Jesorka, A., & Jardemark, K.A multifunctional pipette for localized drug administration to brain slices.Journal of Neuroscience Methods201310.1016/j.jneumeth.2013.08.012
Ainla, A., Gözen, I., Hakonen, B., & Jesorka, A. Lab on a Biomembrane: Rapid prototyping and manipulation of 2D fluidic lipid bilayers circuits. Scientific Reports201310.1038/srep02743
Jansson, E., Trkulja, C., Ahemaiti, A., Millingen, M., Dm Jeffries, G., Jardemark, K., & Orwar, O.Effect of cholesterol depletion on the pore dilation of TRPV1. Molecular Pain201310.1186%2F1744-8069-9-1
Wegrzyn, I., Ainla, A., Jeffries, G., & Jesorka, AAn optofluidic temperature probe. Sensors201310.3390/s130404289
Ainla, A., Jeffries, G., Brune, R., Orwar, O., & Jesorka, A. A multifunctional pipette. Lab on a Chip201210.1039/c2lc20906c
Ainla, A., Xu, S., Sanchez, N., Jeffries, G., & Jesorka, A. Single-cell electroporation using a multifunctional pipette. Lab on a Chip201210.1039/c2lc40563f
Ainla, A., Jansson, E., Stepanyants, N., Orwar, O., & Jesorka, A.A microfluidic pipette for single-cell pharmacology.Analytical Chemistry201010.1021/ac100480f

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Scientist using the BioPen system

Know how and Manufacturing

The core of our technology is based upon controlling the flow of liquids in very small channels, barely visible to the naked eye.

The fabrication of such microminiaturized devices is a delicate process. This requires exceptional skill and understanding of microfluidic phenomena, but also attention to detail and years of accumulated processing know-how.

Expertise, precision and attention to detail set the tone throughout the full fabrication process.

Biopen setup

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Biopen pipette tips

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Biopen delivery zone

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Biopen and cells

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Biopen applications

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Biopen software

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